Fig. 3.

Association of pUL31 with purified intranuclear capsids. Capsids were isolated from the nuclei of Vero cells infected with PRV encoding a myc-pUL31 fusion and either an intact UL34 allele (wild type) or a UL34 deletion allele (ΔUL34) and separated on a linear sucrose gradient by rate-zonal centrifugation. Western blots of gradient fractions probed for myc-pUL31 and VP5 are shown. Fractions are labeled 1 (top of the gradient) to 18 (bottom of the gradient). Densitometries of myc-pUL31 (solid lines) and VP5 (dashed lines) are presented in the bottom panels.