Fig. 5.
Calpain-1 activation affects Rad21 occupancy. (A) Chromatin occupancy of Rad21 and calpain-1 in Jurkat cells. Rad21 and calpain-1 occupancy is shown for nine cohesin binding sites and three cohesin-free sites (CFS). (B) Chromatin occupancy of Myc-tagged WT and del mutant Rad21 in HEK293T cells transfected with control (top) or calpain-1 (duplex A) (bottom) siRNA. Average threshold cycle (CT) values for each primer pair for three independent biological replicates (each run in triplicate) were analyzed. Error bars represent standard errors of the means.