Fig. 7.

The GTPase activity of dynamin-2 is involved in the induction of the processing of p100 in response to activated LTβR. (A) HeLa cells were incubated with DMSO (vehicle) or Dynasore (80 μM) 1 h before stimulation with the agonist (ago) to LTβR for the indicated time period. Total cell extracts were analyzed by immunoblotting for the indicated proteins. (B and C) Experiments were conducted as for panel A with HT1080 and human primary fibroblasts, respectively. (D) HeLa cells were pretreated or not with Dynasore for 1 h followed by a brief stimulation with the ago to LTβR. Cell extracts were immunoprecipitated with an anti-LTβR antibody prior to immunoblotting with anti-TRAF2. (E) The same extracts as in panel A were analyzed for the phosphorylation and degradation of IκBα.