Fig. 3.

CPT induces chromosome breakage in mre11-3 mutants. Chromosome integrity was monitored by PFGE analysis. (A) Analysis of WT and mre11-3 cells. G₁-arrested cells were released into DMSO (D), 50 μM CPT (C), or 0.035% MMS (M). Numbers above the gel indicate time (in min) post-G₁ release; P indicates plug; lane numbers appear below the gel. G₁ (alpha-factor arrested) and G₂ (nocodazole arrested) cells indicate 1n and 2n DNA controls, respectively. The ethidium bromide-stained gel was visualized using UV transillumination. A single representative gel is shown. Observations were confirmed with independent strains. (B) Cell cycle analysis of samples used in panel A. (C) Analysis of mre11-3 and mre11-3 yku70Δ cultures. (D) Cell cycle analysis of samples used in panel C.