Fig. 2.

SPV032 and M029 restore ΔE3L replication in HeLa cells. (A) BHK21 cells were infected with the indicated viruses and collected at 24 hpi. Western blotting was used to detect expression of actin, E3, and HA-tagged SPPV34, YMTV34, SPV032, and M029. (B) BHK21 cells were infected with the indicated viruses at an MOI of 0.5 and collected at 5 and 48 hpi. Virus yields were determined by plaque assays in BHK21 cells. (C) HeLa cells were infected with the indicated viruses at an MOI of 0.5 and collected at 5 and 48 hpi. Virus yields were determined by plaque assays in BHK21 cells. (D) HeLa cells were infected with the indicated viruses at an MOI of 0.01, and eGFP expression was analyzed by fluorescence microscopy at 24 hpi. Microscope settings were kept consistent for all images taken. (E) HeLa cells were infected with each of the indicated viruses at an MOI of 5 and collected at 6 hpi. Western blotting was used to analyze expression of vaccinia virus D12 and H3. The results are representative of three independent experiments. Error bars represent the standard errors of the mean.