Fig. 5.

dsRNA binding capacity of orthologues of the E3 protein. BHK21 cells were infected with the indicated viruses at an MOI of 1 for 16 h. Cells were then collected and lysed in nondenaturing lysis buffer. Lysates were incubated with pIC-conjugated beads, and proteins associated with the beads were isolated using pulldown assays. The presence of actin, D12, E3, HA-tagged SPPV34, YMTV34, SPV032, and M029 were analyzed by Western blotting. The results are representative of four independent experiments.