Fig. 6.

Inhibition of cytokine expression by orthologues of the E3 protein. HeLa cells were infected with the indicated viruses at an MOI of 5 and collected at 9 hpi. The expression of IFN-β (A), TNF-α (B), and IL-6 (C) was quantified by real-time PCR. Shown is the gene expression fold change in infected cells in comparison to mock-infected cells. Actin expression was used as an internal normalization control for total RNA loading. Error bars represent one standard deviation. The results are representative of four independent experiments.