Fig. 7.

Inhibition of the antiviral effects of IFN-β by orthologues of the E3 protein. (A) Huh7 cells were treated overnight with 1,000 U of recombinant human IFN-β/ml. Cells were then infected with the indicated viruses in the presence or absence of 1,000 U of IFN-β/ml and collected at 5 and 48 hpi. Virus yields were determined by plaque assays in BHK21 cells. (B) Huh7 cells were treated overnight with 1,000 U of recombinant human IFN-β/ml. Cells were then infected with the indicated viruses at an MOI of 5 and collected at 6 hpi. The phosphorylation of PKR and eIF2α was analyzed by Western blotting. The results are representative of three independent experiments.