Fig. 7.

Visual identification of eosinophils following lethal challenge. Eosinophils proximal to airways were counted in H&E-stained sections of lung from aged icHC/SZ/61/03-S-infected mice. (A) Representative images of regions counted, with eosinophils highlighted by arrows. (B) Box and whisker counts of eosinophils proximal to airways in icGD03-S-challenged aged mice, with ranges shown by the whiskers. Eosinophils were counted in 4 160-μm² regions proximal to airways; n = 31 fields per group. Both DIV- and DIV-plus-alum-immunized mice had visibly more eosinophils present following challenge than the nonspecific-immunogen groups. ‡, P < 0.0001; t test with Welch's correction.