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Expression and oligomerization of mutant M2 proteins. MDCK cells stably expressing the indicated M2 proteins were analyzed by Western blotting in order to determine the overall expression under reducing conditions (A) and the presence of disulfide-linked oligomers under nonreducing conditions (B). Monomeric M2 and the cell protein loading control, β-actin, are indicated in panel A, and monomers, dimers, and tetramers of M2 are indicated in panel B.
