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Confocal microscopy of HeLa cells infected with vL2-HA. After 9 h, the infected cells were fixed, permeabilized, and stained with a polyclonal primary antibody for A11, L1, or A17 and the mouse anti-HA MAb to L2-HA, followed by goat anti-rabbit IgG and goat anti-mouse IgG coupled to Alexa Fluor 647 and Alexa Fluor 594, respectively, and DAPI. The viral protein (A11, L1, or A17) detected in the third (viral protein) column is shown on the left.
