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. 2011 Dec;85(23):12431–12441. doi: 10.1128/JVI.05573-11

Fig. 3.

Fig. 3.

Colocalization of L2 with calnexin. (A) HeLa cells were infected with vL2-HA. After 2, 4, 6, and 8 h, the infected cells were fixed, permeabilized, and stained with the rabbit anti-calnexin polyclonal antibody and mouse anti-HA MAb to detect L2, followed by goat anti-rabbit IgG and goat anti-mouse IgG coupled to Alexa Fluor 488 and Alexa Fluor 594, respectively, and DAPI. (B) HeLa cells were infected with vL2-HA as in panel A. After 9 h, the infected cells were fixed, permeabilized, and stained with the rabbit polyclonal primary antibody for calnexin, β-COP, or ERGIC-53 (detected in the third cellular protein column as indicated on the left) and the mouse anti-HA MAb to detect L2, followed by goat anti-mouse IgG and goat anti-rabbit IgG coupled to Alexa Fluor 594 and Alexa Fluor 647, respectively, and DAPI. The cells were visualized by confocal microscopy.