Fig. 6.

Localization of L2 by immunogold transmission electron microscopy. BSC-1 cells were infected with vL2-HA. After 20 h, the cells were fixed, cryosectioned, and stained with anti-HA MAb (A to H) or anti-PDI MAb (I and J), followed by rabbit anti-mouse IgG and protein A conjugated to 10-nm gold spheres. (K and L) In addition, 10- and 5-nm gold was used to label L2 and PDI in the same sections. Arrows point to gold particles labeling L2; arrowheads point to PDI. Magnifications are indicated by scale bars at the bottom of each panel.