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Minimal amounts of L2 are associated with virions. HeLa cells were infected with vL2-HA or vA9-HA, and the virions produced were purified through a sucrose gradient (S) and a cesium gradient (C). For each virus, the same amounts of cell extract and purified particles were resolved by SDS-PAGE and analyzed by Western blotting with MAb to the HA epitope tag (α-HA) and polyclonal antibody to L2 (α-L2). Proteins were detected by chemiluminescence.
