Fig. 2.

Localization of ORF2 mutants in Neuro-2A cells. Neuro-2A cells were transfected with 4 μg of the designated plasmids that express a Flag-tagged ORF2 or ORF2 mutants. Cultures were prepared for confocal microscopy at 48 h after transfection as described in Materials and Methods. ORF2⁺ cells were identified using the anti-Flag antibody (red), or DAPI was used to visualize the nucleus (blue). Differential interference contrast (DIC) was used to show the unstained cells. The images are representative of more than 3 experiments.