Fig. 4.

Inhibition of reporter protein synthesis by fish PKR and PKZ. CAB, CO, EPC, COS-7, and 293T cells, seeded in 24-well plates, were cotransfected with the pGL3 plasmid (200 ng) and the expression vectors (200 ng) containing the corresponding inserts indicated by the illustration shown to the left of the bar graph. The K→R mutation that abolishes kinase activity but does not affect dimerization is indicated by a black asterisk. Luciferase activity was normalized by protein contents. A control experiment by using empty vector was set at 100%. Each bar shows the average ± standard error (error bar) for three independent experiments.