Fig. 6.

Effects on GCRV replication by knockdown of PKR or PKZ. (A and B) CAB cells seeded in 24-well plates were delivered with control MO (mock), PKR-MO, PKZ-MO, or PKR-MO plus PKZ-MO (detailed in Materials and Methods) and induced by rIFN at 20 ng/ml for 12 h and subjected to immunoblot analysis. (C to E) The other group of MO-delivered cells were subjected to GCRV infection (each well with 1 ml of a solution with 200 TCID₅₀/ml) for 48 h. While the cells were subjected to Western blot analysis (C and D), the supernatants were collected for detection of virus titers by TCID₅₀ assays (E). The relative levels of eIF2α phosphorylation as determined by quantitative densitometry are shown below the blots in panels B and D.