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. 2011 Dec;85(23):12455–12463. doi: 10.1128/JVI.06023-11

Fig. 2.

Fig. 2.

Seroreactivity of H5N1-negative samples with H5N1-SeroDetect peptides and determination of cutoff values. ELISA conditions were described in Materials and Methods. (A to C) Reactivities of 139 H5N1-seronegative serum/plasma samples from the United States and Vietnam at a 1:100 dilution with the peptides HA2 (488–516) (A), PB1-F2 (2–75) (B), and M2e (2–24) (C) peptides are shown. Values on the x axis are the test specimen ODs with the H5N1 peptide in specific ELISA. The y axis represents the number of serum/plasma samples that exhibited a specific ELISA absorbance reading as shown on the x axis. (D) The cutoff value for each peptide was determined as the mean absorbance + 5 standard deviations obtained with H5N1-seronegative samples.