Table 2.
Summary of H5N1-SeroDetect specificity: no reactivity with serum samples from individuals vaccinated with H5N1 vaccine or individuals with seasonal influenza virus vaccination and infections
| Sample set | Total no. of samples | Country of origin | H5N1-SeroDetect ELISAa |
|||
|---|---|---|---|---|---|---|
| No. of samples reacting positively |
Combined specificity (%) | |||||
| HA2 (488–516) | PB1-F2 (2–75) | M2e (2–24) | ||||
| Samples from seasonal influenza virus vaccination and infection | ||||||
| Prevaccination | 50 | USA | 0 | 0 | 0 | 100 |
| Postvaccination | 50 | USA | 0 | 0 | 0 | 100 |
| Postinfection (seasonal influenza virus) | 50 | USA | 0 | 0 | 0 | 100 |
| Normal human serum samples | 117 | Vietnam | 0 | 0 | 0 | 100 |
| Samples from H5N1 vaccination | ||||||
| Prevaccination | 15 | USA | 0 | 0 | 0 | 100 |
| Postvaccination (H5N1 vaccine) | 128 | USA | 0 | 0 | 0 | 100 |
a
H5N1-SeroDetect reactivity is considered negative if a cutoff value of <1 is obtained in the peptide ELISA. The cutoff value (specimen ELISA OD/control ELISA OD readings) for each peptide was determined as the mean absorbance + 5 standard deviations obtained with H5N1-seronegative samples (Fig. 2) and was 0.09 to 0.11 for the three peptides.