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. 2011 Dec;85(23):12455–12463. doi: 10.1128/JVI.06023-11

Table 2.

Summary of H5N1-SeroDetect specificity: no reactivity with serum samples from individuals vaccinated with H5N1 vaccine or individuals with seasonal influenza virus vaccination and infections

Sample set Total no. of samples Country of origin H5N1-SeroDetect ELISAa
No. of samples reacting positively
Combined specificity (%)
HA2 (488–516) PB1-F2 (2–75) M2e (2–24)
Samples from seasonal influenza virus vaccination and infection
    Prevaccination 50 USA 0 0 0 100
    Postvaccination 50 USA 0 0 0 100
    Postinfection (seasonal influenza virus) 50 USA 0 0 0 100
    Normal human serum samples 117 Vietnam 0 0 0 100
Samples from H5N1 vaccination
    Prevaccination 15 USA 0 0 0 100
    Postvaccination (H5N1 vaccine) 128 USA 0 0 0 100
a

H5N1-SeroDetect reactivity is considered negative if a cutoff value of <1 is obtained in the peptide ELISA. The cutoff value (specimen ELISA OD/control ELISA OD readings) for each peptide was determined as the mean absorbance + 5 standard deviations obtained with H5N1-seronegative samples (Fig. 2) and was 0.09 to 0.11 for the three peptides.