Fig. 2.

Transcomplementation assay used to compare capsid assembly of different AAV serotypes. (A) VP3 of the indicated serotypes was coexpressed with AAP derived from AAV2 in 293T and HeLa cells. Capsid assembly was analyzed by ELISA and immunofluorescence. (B) VP3 and AAP expression constructs, including denotations of the constructs (left) and the expressed proteins (right). Small numbers indicate nucleotide positions relative to the corresponding AAV serotype genome sequences (see Fig. 7 for the accession numbers). Arrows represent the translation start sites of the VP3 and AAP proteins. CMV, human cytomegalovirus immediate-early promoter.