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. 2011 Dec;85(23):12505–12517. doi: 10.1128/JVI.00410-11

Fig. 4.

Fig. 4.

ROS are involved in NLRP3 inflammasome activation by vMyxM013-KO virus. (A and B) THP-1 cells were differentiated with PMA, treated with DPI using the indicated concentrations for 1 h, and then infected with vMyxM013-KO virus at an MOI of 3. Cell supernatants were collected after 6 h to measure the secretion of IL-1β (A) and TNF (B). (C) PMA-differentiated THP-1 cells were mock treated or pretreated with ROS inhibitor DPI (100 μM) for 1 h and then infected with vMyxM013-KO virus. After 6 h cells were loaded with the ROS-sensitive fluorophore H2DCF-DA (10 μM), and fluorescence intensity was measured as described in Materials and Methods. Data are means ± SD of triplicate samples from one experiment and are representative of two or three independent experiments. **, P < 0.01 compared with vMyxM013-KO infection.