Fig. 7.

The MEK-ERK1/2 pathway is involved in activation of both NF-κB and inflammasomes by vMyxM013-KO. (A) THP-1 cells stably transduced with control, ASC, caspase-1, or NLRP3 shRNAs. The cells were differentiated with PMA, treated with U0126 (20 μm) or LY294002 (20 μm) for 1 h, and infected with vMyxM013-KO virus. Cell supernatants were collected after 6 h of infection to quantify the secretion of TNF by ELISA. Data are means ± SD of triplicate samples from one experiment and are representative of three independent experiments (B) Western blots of intracellular phospho-EFK1/2 (pERK1/2), total ERK1/2, and IκBα after treatment with U0126 or LY294002 and infection with vMyxM013-KO.