Table 3.
Genotypic analysis of the GT 2a NS5B gene from PSI-352938- and PSI-353661-selected repliconsa
| Mutation | Codon |
Frequency in replicon |
||
|---|---|---|---|---|
| WT | Mutant | PSI-352938 selected | PSI-353661 selected | |
| S15G | AGC | GGC | 7/10 | 6/8 |
| R222Q | CGA | CAA | 2/10 | 0/8 |
| C223Y | TGC | TAC | 3/10 | 6/8 |
| C223H | TGC | CAC | 7/10 | 2/8 |
| L320I | CTA | ATA | 3/10 | 1/8 |
| V321I | GTA | ATA | 4/10 | 4/8 |
a
Total RNA was extracted from PSI-352938- or PSI-353661-selected cells and no-drug control cells on day 127. JFH-1 NS5B region was reversed transcribed into cDNA and amplified for clonal sequence analysis. Nucleotide changes are underlined.