. 2011 Dec;85(23):12334–12342. doi: 10.1128/JVI.05639-11

Table 6.

Effects of PSI-352938- and PSI-353661-selected mutations in GT 1b replicons^a

Mutation(s)	EC₅₀ fold change		Replication fitness (% relative to WT)
Mutation(s)	PSI-352938	PSI-353661	Replication fitness (% relative to WT)
Single
C223H	ND	ND	0.9 ± 0.49
C223Y	ND	ND	2.2 ± 0.65
V321I	1.3 ± 0.2	1.2 ± 0.2	126.6 ± 8.2
Multiple
C223H/V321I	2.3 ± 0.2	2.6 ± 0.3	21.9 ± 5.5
A15G/C223H/V321I	4.8 ± 1.3	4.8 ± 0.8	10.1 ± 1.1

Effect of amino acid changes at positions 15, 223, and 321 was evaluated using the GT 1b Con1 replicon and a 4-day transient assay. Both replication fitness and sensitivity of the mutated replicon for PSI-352938 and PSI-353661 were examined. Values are averages ± standard deviations from at least three independent experiments performed in duplicate. ND, EC₅₀ could not be determined because the levels of firefly luciferase were too low.