Table 2.
SEC analysis at different concentrations of NSP495-146 and ΔΝ94 purified under different conditionsa
| Purification process | Apparent molecular mass (no. of subunits) |
|||
|---|---|---|---|---|
| SA11ΔΝ94 at concn (mg/ml) of: |
ST3:NSP494-29 at concn (mg/ml) of: |
|||
| 10 | 5 | 20 | 10 | |
| Lysis in acidic pH 5.0 buffer (condition A) | 60.25 (5.51) | 47.86 (4.36) | 34.9 (5.41) | 27.21 (4.2) |
| Lysis in the absence of CaCl2 (condition B) | 56.23 (5.14) | 45.70 (4.08) | 34.9 (5.41) | 29.15 (4.5) |
| Lysis in the presence of CaCl2 (condition C) at concn (mM) of: | ||||
| 0.1 | 57.54 (5.26) | 50.12 (4.58) | ND | ND |
| 1.0 | 54.94 (5.02) | 47.86 (4.38) | ND | ND |
| 10 | 57.54 (5.26) | 48.97 (4.48) | 33.69 (5.21) | 27.86 (4.3) |
| Lysis and purification in the presence of 0.1 mM CaCl2 (condition D) | 54.94 (5.02) | 47.86 (4.38) | 31.75 (4.95) | 25.9 (4.0) |
| Lysis in the presence of 10 mM MgCl2 (condition E) | 54.94 (5.02) | 47.86 (4.36) | ND | ND |
| 27.54 (2.52) | ||||
| Lysis and purification in the presence of 0.1 mM MgCl2 (condition F) | 60.2 (5.51) | 47.89 (4.38) | 32.69 (5.0) | 25.9 (4.0) |
a
Shown are the apparent molecular masses (numbers of subunits) corresponding to the major peaks of the SA11ΔN94 and ST3:NSP495-146 peptides purified under different conditions. Conditions A to F represent different conditions of purification. ND, not determined.