Fig. 3.

G₂ arrest-competent primary Vpr variants can modulate NK cell functions through the NKG2D pathway. (A) CEM.NKR T cells were transduced with GFP-marked Vpr-expressing (or non-Vpr-expressing) lentiviral vectors as indicated in the Fig. 2 legend and stained for ULBP2. Expression levels were gated on GFP⁺ cells. MFI values were obtained after subtraction of signals given by cells stained with a relevant isotype control antibody. (B) GFP⁺ cells were sorted and used as targets in a standard ⁵¹Cr release assay. Purified resting NK cells from healthy donors were used as effector cells at the indicated effector/target ratios. Shown is mean percent specific cell lysis ± SD (triplicate samples) representative of three experiments with different donors. Vertical dotted lines indicated background killing.