RVFV genome organization and expression strategy of the M genome segment. (A) Schematic representation of the RVFV small (S), medium (M), and large (L) genome segments in antigenomic orientation. (Inset) S-based minigenome containing the eGFP gene used in this work. (B) Coding strategy of the M genome segment. The mRNA encoded by the M segment is translated into a polyprotein that is processed into the NSm1, NSm2, Gn, and Gc proteins.