Fig. 5.

Antiviral treatment partially reduces the increased frequency of anti-VACV effector CD8⁺ T cells. B6, IFN-γ^−/−, and Prf^−/− mice were infected with 10⁶ PFU VACV i.p. and treated with 400 μg cidofovir i.p. 2 dpi. At 7 dpi, individual spleens were collected, processed, and analyzed. (A) (Left) Representative flow cytometry plots show the percentages of DimerX staining. (Right) Bar graphs show cumulative data for the percentages of DimerX staining. (B) Total lymphocyte numbers. (C) The number of total CD8⁺ cells increases in infected mice. Data correspond to the same mice as those for which results are shown in Fig. 4 and are representative of two independent experiments. **, P = 0.01; ***, P = 0.001.