Fig. 1.

Protein expression profiles of putative stem cell, hepatoblast, and hepatic progenitor cell markers in the HCV subgenomic replicon-expressing cells (GS5 and FCA4) compared to those of the corresponding controls (GS5-C, Huh7.5, and Huh7) that lack the HCV replicon. (A to D) A 20-μg amount of total lysates was used in each case for Western blotting. (E) Western blot for determination of the DCAMKL-1 level in the Huh7.5 cells transfected with RNAs (5 μg) representing the wild-type JFH1 infectious clone (lane 3) or the mutant version of UFH1 in which RNA polymerase is inactivated (lane 2). Lane 1, mock transfection without RNA. A 40-μg amount of total lysates was used in each case, and an extensive SDS-PAGE analysis was carried out to resolve closely migrating DCAMKL-1 species.