Fig. 11.

siRNA against DCAMKL-1 reduces the expression of the HCV replicon in GS5 cells. (A) Inhibition of the HCV RNA abundance by siRNA against DCAMKL-1 (siDCAMKL-1). siDCAMKL-1 (50 nM and 100 nM) or scrambled siRNA (SCR, 100 nM) were transfected into GS5 cells in triplicates. Total RNAs were extracted and subjected to real-time reverse transcription-PCR. The levels of HCV and DCAMKL-1 RNAs in control untransfected GS5 cells (Cont.) were set as 1 (or 100%). Actin mRNA in each sample was used as internal control for the PCR. The transfections were repeated twice to confirm the results. Error bars represent standard errors of the means. (B) Western blot analysis of cell lysates from siDCAMKL-1-transfected (lanes 3, 4) or untransfected (lane 1) cells as indicated. Lane 2, sample was transfected with scrambled siRNA (SCR).