Fig. 4.

DCAMKL-1-positive cells show CSC traits. (A, B) The GS5 cells were treated with DyLight 549-normal IgG (A, control) or Dylight 549–anti-DCAMKL-1 (B) conjugate and subjected to FACS analysis using lasers that excite both GFP and DyLight 549. (C) Spheroid assay using isolated HCV⁻ DCAMKL-1⁺ cells from the Huh7.5 (bottom) or HCV⁺ DCAMKL-1⁺ cells from the GS5 (top) cell line. The cells (∼100 from each sample) were plated with culture medium containing 50% Matrigel in a 96-well plate. The cells were grown in standard culture conditions, and spheroids were photographed after 4 weeks (a to d).