Fig. 4.

Chemical inhibitors of DNA damage signaling induce cytoplasmic localization of Apoptin in cancer cells. H1299 non-small-cell lung carcinoma cells were infected with Ad-Apwt and then treated with the indicated inhibitors of DNA damage signaling for 18 h. The cells were fixed and stained with anti-FLAG to detect apoptin and DAPI to visualize nuclei. The percentages of cells displaying nuclear localization of Apoptin were determined by microscopy and are graphed on the right. DMSO, dimethyl sulfoxide. Error bars indicate standard deviations.