Fig. 6.

Apoptin nucleocytoplasmic shuttling activity is required to respond to DNA damage. (A) Schematic diagram of N-terminal GFP-tagged Apoptin mutants. (B) H1299 cells were transfected with the indicated GFP-Apoptin constructs and monitored by fluorescence microscopy for GFP (left) and DAPI (center). Merged GFP/DAPI images are shown on the right. (C) Percentages of cells displaying nuclear Apoptin under each of the experimental conditions shown in panel B were determined by microscopy. Error bars indicate standard deviations. *, P < 0.05. WT, wild type.