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Apoptin reduces numbers of DNA damage foci. MRC5 fibroblasts were infected with Ad-Apwt or Ad-LacZ for 48 h and then treated with 10 μM BLM. Following 18 h of treatment, the cells were fixed and stained with anti-FLAG to detect Apoptin and anti-53BP1 to detect DNA damage foci. Nuclei were visualized using DAPI. Percentages of cells displaying DNA damage foci were determined by microscopy and are graphed on the right. Error bars indicate standard deviations.
