Fig. 11.
RNA localizes to SGs in cells expressing G3BPQ326E. (A) Cartoon of reporter RNA containing a hammerhead ribozyme to remove the cap structure and four MS2 binding loops. HeLa cells expressing luciferase reporter and GFP-MS2-NLS in the presence of G3BPQ326E were treated with 0.5 mM arsenite, and reporter localization (green) with initiation factors (red) was monitored. (B) Polyadenylated mRNA (red) was monitored along with TIA1 (green) in cells expressing G3BPQ326E. Cell nuclei were stained with DAPI (blue).