Table 1.

T. maritima KDPG aldolase mutants selected for enhanced catalysis of KHO cleavage.

Name	Mutations	Relative Expression Level
TM 1	G34R/V172A/R190I	++++
TM 2	K9T/G83E	+++
TM 3	*Deletion of first two AA, L16P/V31A/S82C/G83V/F99L/W167R	++
TM 4†	V136M	++
TM 5†	A30V	++
TM 6‡	I52F/V135M/T185A	+
TM 7‡	P109S	+
TM 8‡	N150I	+

⁺⁺⁺⁺100%;

⁺⁺⁺50%;

⁺⁺10%;

⁺5% expression relative to wild-type TMA in TMEDA-pUC plasmid

^†These proteins were selected in a pUC plasmid where the expression of wild-type TMA is 10% of the expression in the TMEDA-pUC plasmid.

^‡These proteins were selected in a pUC plasmid where the expression of wild-type TMA is ~2% of the expression in the TMEDA-pUC plasmid.

^*Signifies a frameshift mutation where expression of the gene is initiated at the third amino acid.