Table 1. Intracellular levels of 8-OHdG and MDA before and after treatment of orbital fibroblasts with 200 μM H2O2.
Oxidative damage | Before treatment (mean±SD) | After treatment (mean±SD) | Induction ratio (%)* (mean±SD) | p-value |
---|---|---|---|---|
8-OHdG(ng/mg DNA) | ||||
Normal |
2.75±0.24 |
3.52±0.20 |
128±5 |
0.005 |
GO |
3.72±0.20 |
5.12±0.32 |
138±11 |
0.005 |
|
p=0.002 |
|
p=0.213 |
|
MDA (nmol/106 cells) | ||||
Normal |
219.80±35.67 |
275.33±40.49 |
126±7 |
0.027 |
GO |
325.92±10.95 |
530.95±62.83 |
163±20 |
0.007 |
p=0.002 | p=0.029 |
*Induction ratio=after H2O2 treatment value /baseline value (%).