Table 2. Intracellular levels of reactive oxygen species in orbital fibroblasts before and after treatment with 200 μM H2O2.
ROS levels | Before treatment (mean±SD) | After treatment (mean±SD) | Induction ratio (%)* (mean±SD) | p -value |
---|---|---|---|---|
O2.- (Relative ratio**) | ||||
Normal |
94.29±1.90 |
102.22±3.47 |
108±3 |
0.039 |
GO |
105.54±3.45 |
118.87±2.92 |
113±2 |
<0.001 |
|
p=0.004 |
|
p=0.055 |
|
H2O2 (Relative ratio**) | ||||
Normal |
105.18±1.79 |
118.81±0.76 |
113±2 |
0.007 |
GO |
123.19±5.84 |
152.97±6.61 |
124±6 |
0.002 |
p=0.004 | p=0.024 |
*Induction ratio=O2.- or H2O2 value after H2O2 treatment / baseline value (%). **Each measurement of cultured orbital fibroblasts was presented as a relative value, which was calculated by taking the intracellular ROS levels of the human skin fibroblasts CCD cell line as 100%.