Table 3. Intracellular levels of antioxidant enzymes in orbital fibroblasts before and after treatment with 200 μM H2O2.
| Activities of antioxidant enzymes | Before treatment (mean±SD) | After treatment (mean±SD) | Induction ratio (%)* (mean±SD) | p-value |
|---|---|---|---|---|
|
Cu,Zn-SOD (μmol/min/mg proteins) | ||||
| Normal |
94.93±16.31 |
100.47±14.15 |
106±5 |
0.132 |
| GO |
86.33±13.02 |
84.14±7.57 |
99±13 |
0.742 |
| |
p=0.470 |
|
p=0.393 |
|
|
Mn-SOD (μmol/min/mg protein) | ||||
| Normal |
65.13±10.68 |
79.79±11.70 |
123±4 |
0.011 |
| GO |
165.70±30.19 |
244.93±45.67 |
148±5 |
0.002 |
| |
p=0.003 |
|
p<0.001 |
|
|
Catalase (μmol/min/mg protein) | ||||
| Normal |
15.47±1.01 |
10.98±1.74 |
71±7 |
0.012 |
| GO |
16.15±1.95 |
6.66±1.04 |
41±3 |
<0.001 |
| |
p=0.613 |
|
p<0.001 |
|
|
GPx (mU/mg) | ||||
| Normal |
239.22±23.24 |
207.63±17.53 |
87±3 |
0.024 |
| GO |
136.42±6.48 |
59.91±11.75 |
44±8 |
<0.001 |
| p<0.001 | p<0.001 | |||
*Induction ratio=enzyme activity after H2O2 treatment/baseline value (%).