Table 2. Mass spectrometric identification of the 2D-PAGE protein spots differentially expressed in the rabbit retina.
| Spot number | Protein name/Swiss-Prot entry (Full chain length) | Theoretical pI;Mr kDa | Fold change RD: Sham | Biological processes |
|---|---|---|---|---|
| 1204 |
α-enolase (434)
ENOA_BOVIN, fragment |
6.99;47.0 |
2.24 |
Glycolysis; Plasminogen activation; Transcription; Transcription regulation |
| 2204/2102/1102 |
ENOA_MOUSE, fragment |
6.99;47.0 |
2.28/2.41/2.42 |
|
| 0502 |
Vimentin (466)
VIME_HUMAN |
5.06;53.5 |
2.51 |
Cell motion |
| 3105/3102/3502 |
Albumin (608)
ALBU_RABIT, N-terminal fragment |
5.67;66.5 |
2.66/2.95/3.19 |
Transport; Cellular response to starvation; Maintenance of mitochondrial location; Negative regulation of apoptosis; Hemolysis of symbiont of host erythrocytes |
| 3301 |
Prohibitin (272)
PHB_HUMAN |
5.57;29.8 |
2.72 |
Negative regulation of cell proliferation; Negative regulation of transcription; Regulation of apoptosis; Signal transduction; Histone deacetylation |
| 9401 |
Fructose-bisphosphate aldolase A
(364)
ALDOA_RABIT |
8.39;39.3 |
0.49 |
ATP biosynthetic process; Fructose 1,6-bisphosphate metabolic process; Regulation of cell shape; Actin filament organization; Striated muscle contraction; Muscle maintenance |
| 1305 |
Transducin-β1 (340)
GBB1_HUMAN |
5.60;37.2 |
0.48 |
Hormone-mediated signaling; Muscarinic acetylcholine receptor signaling; Signal transduction; Ras protein signal transduction |
| 1606 |
ATP synthase subunit β,
mitochondrial (528)
ATPB_BOVIN |
5.00;51.8 |
0.41 |
Protein transport; ATP synthesis coupled protein transport; Regulation of intracellular pH; Angiogenesis |
| 8502/8503 |
Creatine kinase, ubiquitous
mitochondrial (416)
KCRU_BOVIN |
7.31;43.1 |
0.38/0.35 |
Creatine metabolic process |
| 1605 | Tubulin β-2C chain (447) TBB2C_HUMAN | 4.79;49.8 | 0.18 | Cell motion; Microtubule-based movement; Natural killer cell mediated cytotoxicity; Protein polymerisation |
Protein spots 5101, 5703, and 8605 with fold changes of 0.48, 0.43, and 0.38 respectively could not be identified. Biological processes were taken from the Gene Ontology Consortium. Abbreviation: RD represents retinal detachment.