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Protease inhibition with recombinant BPTI. (A) Serial dilutions of recombinant BPTI were incubated with 20 nM bovine chymotrypsin (■), 50 nM human plasmin (▼), and 5 nM bovine trypsin (▲). (B) Recombinant BPTI (filled symbols) and BPTI isolated from bovine pancreas (open symbols) were incubated with 10 nM (circles) and 20 nM (squares) bovine chymotrypsin. Residual amidolytic activity was measured and compared with samples without added inhibitor
