Table 2.
Gene | Ct value range | Slope | R2 | PCR efficiency |
---|---|---|---|---|
APRT | 23-31 | -3.14 | 0.996 | 2.08 |
CSTB | 20-29 | -3.35 | 0.996 | 1.99 |
SPINT3 | 20-29 | -3.26 | 0.998 | 2.02 |
SPINT4 | 21-28 | -3.48 | 0.997 | 1.94 |
The efficiency of PCR experiments was calculated by regression analysis of Ct values generated by serial dilution of epididymis cDNA.