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PCR efficiency
| Gene | Ct value range | Slope | R2 | PCR efficiency |
|---|---|---|---|---|
| APRT | 23-31 | -3.14 | 0.996 | 2.08 |
| CSTB | 20-29 | -3.35 | 0.996 | 1.99 |
| SPINT3 | 20-29 | -3.26 | 0.998 | 2.02 |
| SPINT4 | 21-28 | -3.48 | 0.997 | 1.94 |
The efficiency of PCR experiments was calculated by regression analysis of Ct values generated by serial dilution of epididymis cDNA.
