Table 3.
Relative transcript levels (%) | |||||
---|---|---|---|---|---|
Gene used for normalization | |||||
Tissue | Gene | APRT | σ | CSTB | σ |
Breast | SPINT3 | 0,010 | 0,001 | 0,018 | 0,002 |
SPINT4 | 0,006 | 0,002 | 0,010 | 0,004 | |
Epididymis | SPINT3 | 45 | 5 | 45 | 7 |
SPINT4 | 100 | 14 | 100 | 18 | |
SPINT5 | 0,003 | 0,0005 | 0,003 | 0,0006 | |
Kidney | SPINT3 | 0,0006 | 0,0002 | 0,002 | 0,0004 |
SPINT4 | 0.006 | 0,002 | 0,016 | 0,002 | |
Prostate | SPINT3 | 0,0007 | 0,0005 | 0,001 | 0,0007 |
SPINT4 | 0,001 | 0,001 | 0,002 | 0,002 | |
Seminal vesicle | SPINT3 | 0,056 | 0,017 | 0,053 | 0,014 |
SPINT4 | 0,015 | 0,004 | 0,014 | 0,003 | |
Skin | SPINT3 | 0,029 | 0,007 | 0,053 | 0,013 |
SPINT4 | 0,003 | 0,001 | 0,005 | 0,002 | |
Thyroid | SPINT3 | 0,004 | 0,003 | 0,005 | 0,004 |
SPINT4 | 0,012 | 0,004 | 0,013 | 0,004 | |
Testis | SPINT3 | No signal | No signal | No signal | No signal |
SPINT4 | 0,004 | 0,004 | 0,008 | 0,007 |
Transcript levels of putative Kunitz inhibitors in normal tissues were analyzed by qRT-PCR. The transcript levels were calculated by the comparative ΔΔCt method and normalized with the housekeeping genes APRT and CSTB. Transcript levels are given as % of SPINT4 levels in the epididymis, which was set to 100%. SPINT5 was only detected in the epididymis.