Table 4.

Oligonucleotide primers.

Target gene	Purpose	Primer sequence (5' to 3')
Human SPINT3	RT-PCR and 3' RACE	CCTCTCTCTCGTTTCTCCTGATTCTCACT
Human SPINT3	RT-PCR and 5' RACE	CGCAGCCTCCGTAAGCAAATAACTCACAT
Human SPINT3	qRT-PCR	TCAGGCCTCTCTCTCGTTTCTCCTGATT
Human SPINT3	qRT-PCR	CCCTTTTCCATAGGAAAAGCGCATACAT
Human SPINT4	RT-PCR and 3' RACE	CATCTTCTGCTCATTGAATACCCTGTTATT
Human SPINT4	RT-PCR and 5' RACE	TTACAGCCGGAGAAGACAAAAGTTTCACAT
Human SPINT4	qRT-PCR	TCTTCTGCTCATTGAATACCCTGTTATT
Human SPINT4	qRT-PCR	GAACTTCATAGCAGCTTCCAAAATTCAT
Human SPINT5	3' RACE	CAACTATCCTCTGAAGAAGGGCACCTGT
Human SPINT5	Nested 3' RACE	ATGTGAGCCCTTTGTCTTCAGTGGCTGT
Human SPINT5	5' RACE	GTTTCTGTTGCCTCCACAGCCACTG
Human SPINT5	Nested 5' RACE	CAGCCACTGAAGACAAAGGGCTCAC
Human SPINT5	qRT-PCR	GCTGTGGAGGCAACAGAAACAACTTTAA
Human SPINT5	qRT-PCR	CCTCCTTCTGTCATATTAAGGCCCTATT
Mouse Spint5	RT-PCR and 3' RACE	CCTGCAGGAGTCCGAAGTCAACTCTGCGA
Mouse Spint5	RT-PCR and 5' RACE	TGCAACGGACTTCACAGAGGTATTTAGT
Human APRT	qRT-PCR	CTTGGACTGGGCTGCGTGCTCATCCGAA
Human APRT	qRT-PCR	GTCCTGGCTCCAGGGCGTCTTTCTGAAT
Human CSTB	qRT-PCR	AGTTCCCTGTGTTTAAGGCCGTGTCATT
Human CSTB	qRT-PCR	GTTTTCATGAGGGAGAGATTGGAACACT
Human SPINT3	Cloning in pET32b	GGATCCGAATTCGCGAGACACTATCAAGGATCTCCTCCCAAAT
Human SPINT3	Cloning in pET32b	TCGAGTGCGGCCGCTCAGGTGAACTTGCAGAATTTCTCACAT
Human SPINT4	Cloning in pET32b	CGAATTCGGGTGGTGTTAATAAAATTGCGGAGA
Human SPINT4	Cloning in pET32b	GCGCGGCCGCCTATTGACTTTGGTCCTTTTCAG
Human SPINT5	Cloning in pET32b	CAGTGAATTCCGAATACTGCAACTATCCTCTGAAGAA
Human SPINT5	Cloning in pET32b	TGCAGCGGCCGCTACTTTTCAGTAATACACATTTTTTCACAGA
Bovine BPTI	Cloning in pET32b	GGATCCGAATTCGCCTGACTTCTGCCTAGAGCCTCCATAT
Bovine BPTI	Cloning in pET32b	TCGAGTGCGGCCGCTCACCAGGGCCCAATAGCACCACCACAGGT

The underlined sequences are not present in the target genes.