Table 4.
Target gene | Purpose | Primer sequence (5' to 3') |
---|---|---|
Human SPINT3 | RT-PCR and 3' RACE | CCTCTCTCTCGTTTCTCCTGATTCTCACT |
Human SPINT3 | RT-PCR and 5' RACE | CGCAGCCTCCGTAAGCAAATAACTCACAT |
Human SPINT3 | qRT-PCR | TCAGGCCTCTCTCTCGTTTCTCCTGATT |
Human SPINT3 | qRT-PCR | CCCTTTTCCATAGGAAAAGCGCATACAT |
Human SPINT4 | RT-PCR and 3' RACE | CATCTTCTGCTCATTGAATACCCTGTTATT |
Human SPINT4 | RT-PCR and 5' RACE | TTACAGCCGGAGAAGACAAAAGTTTCACAT |
Human SPINT4 | qRT-PCR | TCTTCTGCTCATTGAATACCCTGTTATT |
Human SPINT4 | qRT-PCR | GAACTTCATAGCAGCTTCCAAAATTCAT |
Human SPINT5 | 3' RACE | CAACTATCCTCTGAAGAAGGGCACCTGT |
Human SPINT5 | Nested 3' RACE | ATGTGAGCCCTTTGTCTTCAGTGGCTGT |
Human SPINT5 | 5' RACE | GTTTCTGTTGCCTCCACAGCCACTG |
Human SPINT5 | Nested 5' RACE | CAGCCACTGAAGACAAAGGGCTCAC |
Human SPINT5 | qRT-PCR | GCTGTGGAGGCAACAGAAACAACTTTAA |
Human SPINT5 | qRT-PCR | CCTCCTTCTGTCATATTAAGGCCCTATT |
Mouse Spint5 | RT-PCR and 3' RACE | CCTGCAGGAGTCCGAAGTCAACTCTGCGA |
Mouse Spint5 | RT-PCR and 5' RACE | TGCAACGGACTTCACAGAGGTATTTAGT |
Human APRT | qRT-PCR | CTTGGACTGGGCTGCGTGCTCATCCGAA |
Human APRT | qRT-PCR | GTCCTGGCTCCAGGGCGTCTTTCTGAAT |
Human CSTB | qRT-PCR | AGTTCCCTGTGTTTAAGGCCGTGTCATT |
Human CSTB | qRT-PCR | GTTTTCATGAGGGAGAGATTGGAACACT |
Human SPINT3 | Cloning in pET32b | GGATCCGAATTCGCGAGACACTATCAAGGATCTCCTCCCAAAT |
Human SPINT3 | Cloning in pET32b | TCGAGTGCGGCCGCTCAGGTGAACTTGCAGAATTTCTCACAT |
Human SPINT4 | Cloning in pET32b | CGAATTCGGGTGGTGTTAATAAAATTGCGGAGA |
Human SPINT4 | Cloning in pET32b | GCGCGGCCGCCTATTGACTTTGGTCCTTTTCAG |
Human SPINT5 | Cloning in pET32b | CAGTGAATTCCGAATACTGCAACTATCCTCTGAAGAA |
Human SPINT5 | Cloning in pET32b | TGCAGCGGCCGCTACTTTTCAGTAATACACATTTTTTCACAGA |
Bovine BPTI | Cloning in pET32b | GGATCCGAATTCGCCTGACTTCTGCCTAGAGCCTCCATAT |
Bovine BPTI | Cloning in pET32b | TCGAGTGCGGCCGCTCACCAGGGCCCAATAGCACCACCACAGGT |
The underlined sequences are not present in the target genes.