Table 1.
PCR amplification primers
| Gene | Primer Sequence | Size (bp) | T(°C) |
|---|---|---|---|
| G3PdH | 5'-TGGCATTGTGGAAGGGCTCATGAC-3' | 188 | 60 |
| 5'-ATGCCAGTGAGCTTCCCGTTCAGC-3' | |||
| NR1 N-ter | 5'-AGTCCAGCGTCTGGTTTGAGATGA-3' | 235(a)/298(b) | 66 |
| 5'-CTCGCTTGCAGAAAGGATGATGAC-3' | |||
| NR2A | 5'-TTCATCTGGGAGCACCTCTT-3' | 402 | 60 |
| 5'-GGCCACAAATGTTTGGAGTT-3' | |||
| NR2B | 5'-GGCATTGCTATCCAAAAGGA-3' | 449 | 60 |
| 5'-GACAGGTTGGCCATGTTCTT-3' | |||
| NR2C | 5'-GCACACCCACATGGTCAAGTTC-3' | 327 | 60 |
| 5'-CTGCTCATCACCTCATTCTTCT-3' | |||
| NR2D | 5'-GCAATACCCACCTCTGAAGTTT-3' | 350 | 60 |
| 5'-CCAGCATCTCAATCTCATCGTC-3' | |||
| NR3A | 5'-GGTTCCCTGCGGAAAGAGAAGC-3' | 310/370 | 60 |
| 5'-TACCATGAGCTGCTGGGGTCCC-3' | |||
| NR3B | 5'-GGTTGGGGACAAAACCTTTGAGG-3' | 287 | 60 |
| 5'-CGGTGAGCAGCTTGCAGTCC-3' | |||
| NR1 C-ter | 5'-ATGCCCCTGCCACCCTCACTTTTG-3' | 381(NR1.1)/270(NR1.2) | 70 |
| 5'-GCAGCTGGCCCTCCTCCCTCTCA-3' | |||
| NR1 C-ter | 5'-TACAAGCGACACAAGGATGC-3' | 241(NR1.3)/132(NR1.4) | 60 |
| 5'-CGAGGGATCTGAGAGGTTGA-3' |