Table 7.
Expression analysis of common bean contigs.
| 454 Contig Number | 2-ΔΔCT Values | Functional Annotation | Primer Sequences | ||
|---|---|---|---|---|---|
| Flower | Pod | Root | |||
| contig04711 | 3.43 ± 0.04 | -2.20 ± 0.09 | -0.82 ± 0.26 | Alcohol dehydrogenase [Prunus armeniaca] | 5'-ATA TGC CTC TGT CTT GGC AGG AGT-3' 5'-ACC TCG GGC AAT AGC ATT GAC TCT-3' |
| contig07734 | 2.16 ± 0.19 | 1.52 ± 0.07 | -0.45 ± 0.1 | Phosphoenolpyruvate carboxylase kinase [Glycine max] | 5'-AGA ATG TGC GAA ACG CTG AAG ACG-3' 5'-AGG ATG GAA ACA CCG GAA GAT GGT-3' |
| contig08043 | 3.78 ± 0.18 | -1.46 ± 0.17 | -0.28 ± 0.18 | Starch synthase III [Phaseolus vulgaris] | 5'-AAG AAC TTG CTA GGG TGC AAG CTG-3' 5'-CTT TGC AGC TCT GTC TGC CTC AAT-3' |
| contig08830 | -5.22 ± 0.14 | 6.91 ± 0.17 | -1.24 ± 0.04 | WRKY35 [Glycine max] | 5'-TCA GCC TTG ACC TTG GTA TGG GAA-3' 5'-TTG CTG GTA TGA GCT TGG CTG TCA-3' |
| contig01300 | * | -10.28 ± 0.07 | 1.32 ± 0.26 | MADS box protein SEP3 [Lotus corniculatus var. japonicus] | 5'-AAT TGC TCA TGC TTG GAC CTG CTG-3' 5'-TGA AGA CAT GGG ATA TGG CAG GCA-3' |
| contig13083 | -0.69 ± 0.17 | 2.55 ± 0.12 | 1.49 ± 0.11 | 4-hydroxyphenylpyruvate dioxygenase [Glycine max] | 5'-TTA TGC CAA CCT TCA CAA GCG TGC-3' 5'-TGC CCT GAT CGT CTC TGT CAA CAA-3' |
| contig14749 | 6.66 ± 0.08 | 0.17 ± 0.09 | 3.07 ± 0.27 | Senescence-associated nodulin 1A [Glycine max] | 5'-TTC TTC TTC CCT GCA CAC GAC ACT-3' 5'-TTG CTG CCC TTT CTA CGG ACA AGA-3' |
| contig17174 | -1.41 ± 0.24 | -6.58 ± 0.07 | 3.63 ± 0.12 | Dihydroflavonol-4-reductase 2 [Glycine max] | 5'-TGG TAG CCT CAT GCG AAC AGC ATA-3' 5'-AGG CCA GTT CGT GCA CTT AGA TGA-3' |
| contig14217 | -1.88 ± 0.14 | 9.86 ± 0.1 | 1.15 ± 0.04 | NAC domain protein NAC1 [Phaseolus vulgaris] | 5'-TGG GTG CCC TTC CTT GAT AGA ACA-3' 5'-TGC AAC AGG GTT ACG CAC AAA TCG-3' |
| contig20010 | 6.07 ± 0.05 | 0.36 ± 0.08 | 1.34 ± 0.27 | ABC transporter family protein [Arabidopsis thaliana] | 5'-ACA ACC TTT GTT TCA GCA CGG AGC-3' 5'-GAG ACA TGG GCA ACT CAT TTG GCA-3' |
| contig28207 | 1.40 ± 0.19 | -2.44 ± 0.12 | -2.38 ± 0.1 | Late embryogenesis abundant protein Lea14-A, putative [Ricinus communis] | 5'-TGA CAG TCT GTT CTC CGT GTG CAT-3' 5'-TAA AGA ACC CAA ATC CGG TGC CGA-3' |
| contig28548 | * | -1.99 ± 0.06 | -0.33 ± 0.1 | nodulin-26 | 5'-TTG GTC CAG GTC CAG CTA ACA ACA-3' 5'-CCC ATC GCC ATT GGT TTC ATC GTT-3' |
| contig29672 | 1.51 ± 0.24 | 3.16 ± 0.11 | 1.01 ± 0.07 | glutathione S-transferase GST 19 [Glycine max] | 5'-AGC TCT TCA AGG ACA CTG AGC CAA-3' 5'-AAA GGC TGT GGA TGC TGC ACT AGA-3' |
| contig28742 | -0.84 ± 0.21 | -6.13 ± 0.07 | -4.19 ± 0.1 | Fructokinase-like protein [Cicer arietinum] | 5'-TGA GTA TTT GCT GAC GCG CTT CCT-3' 5'-GCA CAC CTG AAG GCA ATG GAA GTT-3' |
| contig28845 | -0.98 ± .014 | 0.51 ± 0.1 | -2.96 ± 0.04 | NAC domain protein [Glycine max] | 5'-TGG TGT GGT CCT GCA GAG TGT AAA-3' 5'-AAC GTC GGT GAT TGG GAG GAG AAA-3' |
| contig28932 | 4.83 ± 0.24 | 4.73 ± 0.07 | 3.81 ± 0.07 | Nodule-enhanced protein phosphatase type 2C [Lotus japonicus] | 5'-AAC GTC GGT GAT TGG GAG GAG AAA-3' 5'-CTT GCT GCT TCG CTT TGT CAC TGT-3' |
| contig28932 | -4.04 ± 0.16 | 7.80 ± 0.12 | -5.34 ± 0.12 | nodule-enhanced protein phosphatase type 2C [Lotus japonicus] | 5'-AAC GTC GGT GAT TGG GAG GAG AAA-3' 5'-ATC CCT CTC TCC TTC GCA GCA AAT-3' |
| contig30192 | 1.61 ± 0.22 | * | 0.58 ± 0.11 | WRKY54 [Glycine max] | 5'CAA CAC ACA CAT CCA AGC CCA GTT3' 5'TGG TTC TGC TGC TGC TGA TAC TGT3' |
| contig30958 | 1.17 ± 0.15 | 11.91 ± 0.11 | 3.89 ± 0.08 | WRKY27 [Glycine max] | 5'ACG GAA ACT CTG AGA GCA GCT CAA3' 5' TGC TTC CGT CCT CAC GTA AAC TCT3' |
| contig32565 | -8.41 ± 0.15 | -10.63 ± 0.11 | 0.90 ± 0.37 | MADS transcription factor [Glycine max] | 5'-TGC CTC ACC TAG CAA GTG TTC CTT-3' 5'-AGA TCT TGG CCC TCT AAG CAG CAA-3' |
| contig32665 | 1.26 ± 0.24 | 4.39 ± 0.09 | 0.33 ± 0.07 | Transcriptional factor NAC11 [Glycine max] | 5'-AAT GTG GTC TGA GGA GGT GGT GTT-3' 5'-ATG CTC TAA CTT CAG CGG AGG CAA-3' |
| contig32781 | 1.33 ± 0.17 | 1.82 ± 0.12 | -2.89 ± 0.12 | WRKY23 [Glycine max] | 5'GCA TGT TGC TGT CAG GGT CAA TGT3' 5'TGG TGC TGA AGC TGA AAG TGT TGC3' |
| contig33251 | -0.09 ± .019 | -3.93 ± 0.06 | -4.10 ± 0.1 | Sucrose synthase [Vigna angularis] | 5'-ACG GCT AGT TTC CTT GTG GGA GAA-3' 5'-TCT CAC ACA GCT TTC ACC CTT CCT-3' |
* Data not available (inadequate replicates)