Table 1.

Derivation of haploid mouse ESC lines

Exp. No	derivation protocol	Names of ESC lines used in study	genetic background	Oocytes activated	number of blastocysts	ESC lines obtained	ESC lines with haploid contribution (max. % haploid before sorting)
1	2i / immunosurgery	HAP-1 to 6 †	B6CBAF1	132	30	27	6 (>60%)
2	2i	HAP-7	B6CBAF1	22	10	5	1 (>15%)
3	2i	HTG-1 to 3 ‡	mixed TG*	50	32	3	3 (>90%)
4	KSR / immunosurgery	HAP-8 to 13	B6CBAF1	273	48	22	6 (>10%)
5	2i	H129B6-1 to 5	129B6F1	250	37	8	5 (>10%) 5
6	2i	HTX-1	mixed TG**	70	11	1	1 (>40%)
7	2i	H129-1 to 3	129Sv	140	13	10	3 (>60%)

^†Contribution to chimeric mice was confirmed for the HAP-1 and HAP-2 haploid ESC lines.

^‡Contribution to chimeric mice was confirmed for the HTG-1 haploid ESC line.

^*Derived from ROSA26^nlsrtTA LC1 Xist^2LOX homozygous female mice.

^**Derived from ROSA26^nlsrtTA tetOPXist homozygous female mice³⁰.