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. Author manuscript; available in PMC: 2011 Nov 6.
Published in final edited form as: Cell. 1997 Dec 26;91(7):1007–1019. doi: 10.1016/s0092-8674(00)80491-2

Figure 4. Fluorescence In Situ Hybridization (FISH) to Mitotic Metaphase Chromosomes with AATAT (A) and AAGAG (B) Satellites, and transposable elements 412 (C) and F (D).

Figure 4

DAPI stained (blue) neuroblast squashes are superimposed with the hybridization signals (pseudocolored FITC fluorescence in red). The normal chromosomes are indicated, as is the γ1230 minichromosome that serves as an internal quantitation control. Note that only one copy of γ1230 is present in (A) and (B) (outcrossed stocks), while five copies are seen in (C) and two in (D) (inbred stocks). None of these probes hybridizes to all centromeric regions (arrows); for example, the centromeric regions of the second chromosomes in (A) lack AATAT satellite signal, the centromeric regions of the third chromosomes in (B) are not labeled by the AAGAG probe, and the fourth chromosomes contain no 412 signal. None of these sequences are confined only to the centromeric regions.