Fig. 2.
Co-administration of LPS injection with hypoxia-ischemia (H/I) exacerbated white matter injury. MBP staining of animals killed at 96 h after H/I with vehicle (PBS) or different doses of LPS showed that 1 or 3 mg/kg LPS, but not 0.1 mg/kg LPS, combined with UCL/hypoxia (6% O2, 30 min), caused myelin loss both contralateral and ipsilateral to the ligation (A). O1 staining of same animals showed similar results on oligodendrocyte loss both contralateral and ipsilateral to the ligation (C). Statistical analysis of the semi-quatitative scores of MBP and O1 staining showed that LPS co-administration (1 or 3 mg/kg) with UCL/hypoxia (30 min) significantly increased the white matter injury bilaterally, as compared to UCL/hypoxia (30 min) (n=6–9, *p<0.01 ** p<0.001) (B, D).